THE EFFECT OF WATER EXTRACT OF COLA NITIDA POD ON LIPOPROTEIN CONCENTRATIONS OF ALBINO WISTAR RATS
CHAPTER ONE                                       
1.0 Introduction -                                       
CHAPTER TWO                                       
2.0    Literature Review -                             
2.1    Kola Nut Powered (Cola Nitida) -                   
2.2    Kola pod components -                             
2.3    Geography and distribution -                        
2.4    Lipoprotein -                                  
2.5    High cholesterol diagnosis -                        
2.6    Low cholesterol -                                  
2.7    Changes during circulation -                        
2.8    Liver -                                       
CHAPTER THREE    Materials and methods              
3.1    Identification and Extraction plant material -              
3.2    Phytochemical Analysis -                             
3.3    Experimental Animal model -                        
3.4    Collection of Blood samples -                        
3.5    Lipid profile Analysis -                             
CHAPTER FOUR    Results                             
Phytochemical composition of samples -                   
CHAPTER FIVE         Discussion and conclusion              
5.1    Discussion - -                                  
5.2    Conclusion --                                  
 Reference -
ABSTRACT
Cola nitida has been in use in the eastern parts of Nigeria for the management of certain ailments and as an aphrodisiac in the management of some sexual dysfunctions. However the effects of this extract in some health parameters in human objects have not been reported. This work, then, was aimed at investigating the effect of water extracts of kola pods on lipoprotein concentrations on albino wistar rats.
 Rats were used in this study and they were divided into several groups and fed with a high calorietoincreasethe food ( blood lipoprotein concentration in the rat models. The effect of the water extracts of the sample used was compared with a known drug and the results statistically compared to ascertain the effect of the water extract on the rat models.
The results of this work indicate that water extracts of cola nitida pods can be used in the management of mild lipoprotein dysfunctions thus substantiating the reasons for its use in sexual erectile functions.
CHAPTER ONE
1.1            INTRODUCTION
Cola Nitida has been used in folk medicine as an aphrodisiac,  an appetite suppressant, to treat morning sickness, migraine headache, and indigestion (Esimone et al., 2007). It has also been applied directly to the skin to treat wounds and inflammation (Newall et al.,1996)on the teeth and gums. There has also been speculations as to its use in the treatment of peptic ulcer, however, in Nigeria there is no record of the incidence of peptic ulcer.
According to Esimone (2007), cola nitida tree is native to West Africa. Cola nuts are obtained from cola trees. Cola nitida belongs to the genus cola and family steriliaceae. They are commonly used to counteract hunger and thirst; in some cases it is used to control vomiting in pregnant woman and also as a principal stimulant to keep awake and withstand fatigue by students, drivers, and other menial workers.
Lipoproteins are found in the liver and cells. The liver is the largest organ inside the body. The liver is dark reddish-brown and consists of two main lobes. There are over 300 billion specialized cells in the liver that are connected by a well organized system of “bile”            ductssels calledandthe biliarybloodsystem.  ves
CHAPTER TWO
LITERATURE REVIEW
 Cola husk pod has great potentials as feed ingredients (Hamzat et al., 2003). Cola tree is a tropical tree which belongs to the family of steriliaceae. It is mostly common in the rain forest region of West Africa. This crop is of socio-economic importance; there is a variety of kola species, out of which cola nitida and cola accuminata are of major economic and social importance in Nigeria. (Babafunde et al.,2001.)
 According to Russelu (2005) the tree is a robust tree usually from 12m high. In South Western Nigeria where large plantation is established, I discovered that kola nitida pod husk shares several similarities with coco pod husk; both have high crude protein and low crude fibre content. Kola pod husk contains the highest value of calcium (0.60%), chloride ion (0.44%) and molybdenum (9.4ppm) (lowest value of this nutrient is observed in kola test (0.7ppm). The seeds of both cola nitida and cola accuminata, its nutritive quality have been reported cogutuga.
2.1                KOLA POD COMPONENTS
 The kola pod components were found to have supportive effect on fat accumulation. Sayami et al., 2010, described that 2% caffeine powder suppresses body weight gain and fat accumulation. It also reduces triacylglycerol (TG) and total cholesterol (TC) in the liver and as well as (TG) in serum, without reducing food intake (in mice). As a result, it was shown that the combination of caffeine and catechin might be the most effective of all green tea components in suppressing fat accumulation. Body fat gain in mice fed with a high-fat diet was significantly suppressed. Catechins and MRNA expressions of acyl-con oxidase (ACO) and medium chain acyl-con dehydrogenase (MCAD), which are related to B-oxidation within the liver were upregulated. It was also demonstrated that weight gain, body fat, accumulation, TG and TC in the liver, and TG in serum were suppressed in rats fed on normal diet and water containing 0.5% catechins. Additionally, a diet containing 1% catechins decreased TG levels and enzymatic activities of fatty acid synthesis (F & S) on enzymes involved in fatty acid synthesis in rat liver. Rats fed with a high-fat diet with 0.05% or 0.14% caffeine demonstrated a reduction in body fat mass as well as hepatic and serum TC and TG levels. Moreover, serum catecholamine concentrations were increased in rat that were orally administered 5mg/kg of caffeine.
 Taxonomy
 Class:           Equisetopsida
Subclass:                 Magnolidae
Superorder:            Rosanae
 Order:          Malvales
 Family:        Malvaleae
 Genus:         cola
 Common name (s):          Kola  nut,  cola,  kola,  siffer  kola  (English)
 Abata cola, Gbaja cola, goro cola, labozlic kola (traditional names).
 2.3            GEOGRAPHY & DISTRIBUTION
Cola nitida is native to West Africa (from Guinea to Ghana)  and has been introduced throughout the forested areas of West and Central  Africa.  Commercial  crops  are  grown  mainly  in  Nigeria, Ghana, Cote d’ivoireSierra Leoneandand also to some extent in India, Brazil and Jamaica.
 2.4            LIPOPROTEIN
A  lipoprotein  is  a  biochemical  assembly  that  contains  both proteins and lipids. Many enzymes, transporters, antigens, adhesions, structural proteins and toxins are lipoproteins. The trans membrane proteins of the mitochondrion and the chloroplast, and bacterial lipoprotein functions of lipoprotein particles is to transport water insoluble lipids (fats) and cholesterol around the body in the blood. The various types of lipoprotein in the body enable fats to be carried in the blood stream. The lipoproteins have hydrophilic groups of phospholipids, cholesterol and apoproteins directed outward. Such characteristics make them soluble in the salt water based blood pool. Triglyceride – fats and cholesterol esters are carried internally, shielded from the water by the phospholipids monolayer and the